Structural Analysis of -Carrageenan Sulfated Oligosaccharides by Positive Mode Nano-ESI- FTICR-MS and MS/MS by SORI-CID
نویسندگان
چکیده
Structural analysis of sulfated oligosaccharides from -carrageenan of up to ten residues (MW 2 kDa) was successfully carried out by positive mode nano-ESI-FTICR-MS together with MS/MS using sustained off-resonance irradiation-collision induced dissociation (SORI-CID). Glycosidic bond cleavage reactions via the Band Y-types of fragmentation were observed and enabled complete sequencing of the oligosaccharide samples. The positions of the labile sulfate substituents were observable using SORI-CID, enabling the determination of the sequence of the sulfated residues. (J Am Soc Mass Spectrom 2006, 17, 96–103) © 2005 American Society for Mass Spectrometry
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